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Abstract:
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Vancomycin is a hospital antibiotic used in Gram+ bacteria infection. The recommended
plasma concentration is 15 - 20 µg/mL, values above can cause nephrotoxicity and ototoxicity. This,
added to the intra-individual pharmacokinetic variations, makes it an excellent candidate for
therapeutic monitoring, which, through the measurement and interpretation of plasma levels, ensures
effective and safe individualized doses. So, this study is a method validation for the determination of
vancokinemia by high performance liquid chromatography with a UV detector and the application in a
Santa Catarina - Brazil hospital. The method obtained linearity between 5 – 100 µg/mL with a Lower
Limit of Quantitation of 5 µg/mL and Limit of Detection of 1.45 µg/mL, presented adequate
repeatability, intermediate precision, intraday/interday accuracy according to legislation and no matrix
effect. Samples were stable for 24 hours at room temperature, 48 hours post-processing, up to 8
months stored and up to 2 freeze/thaw cycles. The method was applied to four patients, with 12
determinations performed, 66% indicated the need for a change in dose. In conclusion, the method
was successfully validated and applied in the hospital, with challenges to be resolved in the continued
use of the method. |
